Lexi & Lottie: Trusty Twin Detectives follows the adventures of 12-year-old identical twin sisters, Lexi and Lottie, who use their unique bond and detective skills to solve mysteries and crimes in their small town of Appleton and its Animal Rehabilitation Park. With the help of their pet mouse, a nose for clues, and their journalist friend Fred, the twins prove that two heads are better than one, especially when it comes to cracking cases and bringing culprits to justice. But being detectives isn't their only job - they also have to navigate the challenges of school, family, and growing up.

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Some TV shows that have similar genres and were produced around the same time as Lexi & Lottie: Trusty Twin Detectives include Dora the Explorer, The Adventures of the Young Marco Polo, Pokémon Master Journeys, Spirit Riding Free: Riding Academy, Pokémon: The Arceus Chronicles, Pokémon Concierge.